Adducin alpha (pSer59) antibody

Details for Product No. ABIN649256
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
pSer59
(3), (1), (1)
Reactivity
Human
(5), (4), (3)
Host
Rabbit
(5)
Clonality
Monoclonal
Application
Western Blotting (WB)
(5), (3), (2), (1)
Pubmed 1 reference available
Catalog no. ABIN649256
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 59.
Background Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and Beta, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Synonyms: ADD1, ADDA, Alpha-adducin, Erythrocyte adducin subunit alpha
Molecular Weight 115 kDA
Gene ID 118
UniProt P35611
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits, α, and β,, or α, and γ, subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Adducin alpha Antibody Phospho (pS59) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kuhlman, Hughes, Bennett et al.: "A new function for adducin. Calcium/calmodulin-regulated capping of the barbed ends of actin filaments." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 7986-91, 1996 (PubMed).

Hosts (5)
Reactivities (5), (4), (3)
Applications (5), (3), (2), (1)
Epitopes (3), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top