Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
Synonyms
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ... show more
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN649267
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Akt1
Gene ID 207
UniProt P31749
Description Other names: AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, C-AKT
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody can detect Akt1 and Akt3. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Molecular Weight 60 kDA
Comments

Background: Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.
Synonyms AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details
Publications Alternatives Variants back to top

Application Notes The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:10~80 FC: ~~ 1:10~50 ICC: ~~ 1:1000 IHC: ~~ 1:1000~1:2500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Akt1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Cross, Alessi, Cohen et al.: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts Rabbit (358), Mouse (95), Sheep (6), Goat (3), Chicken (1)
Reactivities Human (452), Mouse (Murine) (277), Rat (Rattus) (272), Chicken (114), Dog (Canine) (111), Cow (Bovine) (93), Sheep (Ovine) (88), Pig (Porcine) (83), Rabbit (37), Monkey (21), Chimpanzee (5), Horse (Equine) (5), Cat (Feline) (4), Chemical (3), Xenopus laevis (2), Arabidopsis thaliana (1), Frog (1)
Applications Western Blotting (WB) (335), ELISA (219), Immunofluorescence (IF) (151), Immunohistochemistry (IHC) (115), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (84), Flow Cytometry (FACS) (77), Immunoprecipitation (IP) (38), Immunocytochemistry (ICC) (19), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Dot Blot (Dot) (7), Fluorescence Microscopy (FM) (5), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (DB) (1)
Conjugates Biotin (12), Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (7), HRP (5), PE (5), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), APC (1), Agarose Beads (1), Atto 594 (1), CF (1), Sepharose (1)
Epitopes pSer473 (39), pThr308 (28), C-Term (21), pTyr474 (19), pSer473,pTyr474 (18), N-Term (15), pThr450 (12), Internal Region (8), pSer129 (8), Ser473 (7), pTyr326 (7), Thr308 (6), pSer124 (4), pThr34 (4), Ser124 (3), Ser129 (3), Thr450 (3), pSer246 (3), AA 460-480 (2), Ser246 (2), pSer473,Internal Region (2), AA 2-480 (1), AA 302-311,pThr308 (1), AA 460-480,C-Term, pSer473 (1), AA 464-477 (1), AA 74-89 (1), AA 80 (1), C-Term,Thr450 (1), N-Term,Gly37 (1), Phosphospecific (1), Tyr326 (1), pSer269 (1), pSer308 (1), pThr308,Internal Region (1), pThr500 (1), pThr72 (1), pTyr204 (1), pTyr312,pTyr316,pTyr315 (1)