V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody

Details for Product No. ABIN649269
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
C-Term
(136), (90), (64), (61), (60), (49), (37), (33), (29), (20), (19), (17), (17), (17), (16), (14), (10), (10), (9), (8), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(958), (558), (525), (165), (155), (117), (94), (24), (18), (4), (3), (1), (1), (1), (1)
Host
Rabbit
(822), (144), (11), (5), (3), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(34), (28), (27), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(740), (435), (311), (162), (142), (140), (117), (70), (67), (41), (11), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
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Catalog No. ABIN649269
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Specificity A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with mouse, rat and bovine, based on sequence homology.
Alternative Name Akt1
Background Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.
Synonyms: AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, C-AKT
Molecular Weight 59 kDA
Gene ID 207
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Application Notes IHC: = 1:100, WB: = 1:1000
Comment

Background: Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Akt1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Burgering, Coffer: "Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction." in: Nature, Vol. 376, Issue 6541, pp. 599-602, 1995 (PubMed).

Cross, Alessi, Cohen et al.: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996 (PubMed).

Cardone, Roy, Stennicke et al.: "Regulation of cell death protease caspase-9 by phosphorylation." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1318-21, 1998 (PubMed).

Hajduch, Litherland, Hundal: "Protein kinase B (PKB/Akt)--a key regulator of glucose transport?" in: FEBS letters, Vol. 492, Issue 3, pp. 199-203, 2001 (PubMed).

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