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Tubulin, alpha 1B (TUBA1B) antibody

Antigen

Tubulin, alpha 1B (TUBA1B)
Synonyms Tuba2, MGC102558, MGC117866, MGC118411, TUBA2, TUBA8, TUBA1B, TUBA3D, TUBA3, K-ALPHA-1, tuba, tuba1, k-alpha-1, RGD1565476
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649289
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Alpha-Tubulin
Gene ID 10376
UniProt P68363
Description Other names: TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain,Alpha-tubulin ubiquitous,Tubulin K-alpha-1
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen.
Molecular Weight 50 kDA
Comments

Background: Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:50000~200000 IP: ~~ 1:10~100 FC: ~~ 1:100~500 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Alpha-Tubulin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Cleveland, Lopata, MacDonald et al.: "Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes." in: Cell, Vol. 20, Issue 1, pp. 95-105, 1980 (PubMed).

Westermann, Weber: "Post-translational modifications regulate microtubule function." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 938-47, 2003 (PubMed).

Wang, Nogales: "Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly." in: Nature, Vol. 435, Issue 7044, pp. 911-5, 2005 (PubMed).