|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Tubulin, alpha 1B (TUBA1B) antibody
|Synonyms||Tuba2, MGC102558, MGC117866, MGC118411, TUBA2, TUBA8, TUBA1B, TUBA3D, TUBA3, K-ALPHA-1, tuba, tuba1, k-alpha-1, RGD1565476|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain,Alpha-tubulin ubiquitous,Tubulin K-alpha-1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen.|
|Molecular Weight||50 kDA|
Background: Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:50000~200000 IP: ~~ 1:10~100 FC: ~~ 1:100~500 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Alpha-Tubulin Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Cleveland, Lopata, MacDonald et al.: "Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes." in: Cell, Vol. 20, Issue 1, pp. 95-105, 1980 (PubMed).
Westermann, Weber: "Post-translational modifications regulate microtubule function." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 938-47, 2003 (PubMed).
Wang, Nogales: "Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly." in: Nature, Vol. 435, Issue 7044, pp. 911-5, 2005 (PubMed).