anti-alpha Tubulin antibody (TUBA1)

Details for Product anti-TUBA1 Antibody No. ABIN649289
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human, Rat (Rattus)
(99), (46), (42), (23), (21), (16), (8), (8), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(80), (34), (14), (7), (2)
Clonality
Monoclonal
Conjugate
This alpha Tubulin antibody is un-conjugated
(7), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(116), (40), (36), (33), (32), (28), (27), (10), (7), (4), (2), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
Shipping to United States ( )
Availability Please contact our customer support.
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Specificity A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen.
Alternative Name Alpha-Tubulin (TUBA1 Antibody Abstract)
Background Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).
Synonyms: TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain,Alpha-tubulin ubiquitous,Tubulin K-alpha-1
Molecular Weight 50 kDA
Gene ID 10376
UniProt P68363
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB: = 1:50000-200000 ,IHC: = 1:250-500
Comment

Background: Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Alpha-Tubulin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Product cited in: Liu, Zhou, Zhou et al.: "The ubiquitin ligase CHIP inactivates NF-κB signaling and impairs the ability of migration and invasion in gastric cancer cells." in: International journal of oncology, Vol. 46, Issue 5, pp. 2096-106, 2015 (PubMed).

Background publications Wang, Nogales: "Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly." in: Nature, Vol. 435, Issue 7044, pp. 911-5, 2005 (PubMed).

Westermann, Weber: "Post-translational modifications regulate microtubule function." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 938-47, 2003 (PubMed).

Cleveland, Lopata, MacDonald et al.: "Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes." in: Cell, Vol. 20, Issue 1, pp. 95-105, 1980 (PubMed).

Validation Images
Catalog No. ABIN649289
481.43 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?