alpha Tubulin (TUBA1) antibody

Details for Product No. ABIN649289
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Antigen
Synonyms K-ALPHA-1, TUBA2, mec-12, tuba4
Reactivity
Human, Rat (Rattus)
(73), (32), (30), (21), (17), (13), (7), (6), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(65), (22), (12), (7), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(93), (36), (31), (26), (22), (20), (16), (9), (6), (5), (2), (1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN649289
450.00 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Specificity A synthetic peptide corresponding to residues near the N-term of human ?-Tubulin was used as immunogen.
Alternative Name Alpha-Tubulin
Background Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).
Synonyms: TUBA1B, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain,Alpha-tubulin ubiquitous,Tubulin K-alpha-1
Molecular Weight 50 kDA
Gene ID 10376
UniProt P68363
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes WB: = 1:50000-200000 ,IHC: = 1:250-500
Comment

Background: Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Alpha-Tubulin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Cleveland, Lopata, MacDonald et al.: "Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes." in: Cell, Vol. 20, Issue 1, pp. 95-105, 1980 (PubMed).

Westermann, Weber: "Post-translational modifications regulate microtubule function." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 12, pp. 938-47, 2003 (PubMed).

Wang, Nogales: "Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly." in: Nature, Vol. 435, Issue 7044, pp. 911-5, 2005 (PubMed).

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