AMPK beta I antibody

Details for Product No. ABIN649296
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human, Rat (Rattus)
Host
Rabbit
Clonality
Monoclonal
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 3 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649296
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen.
Background The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α, (63 kDa), Beta (38 kDa) and γ, (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).
Synonyms: PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1
Molecular Weight 38 kDA
Gene ID 5564
UniProt Q9Y478
Research Area Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes IHC: = 1:100-250, WB: = 1:5000
Comment

Background: The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α, (63 kDa), β, (38 kDa) and γ, (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment AMPK beta I Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Davies, Hawley, Woods et al.: "Purification of the AMP-activated protein kinase on ATP-gamma-sepharose and analysis of its subunit structure." in: European journal of biochemistry / FEBS, Vol. 223, Issue 2, pp. 351-7, 1994 (PubMed).

Woods, Cheung, Smith et al.: "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 17, pp. 10282-90, 1996 (PubMed).

Thornton, Snowden, Carling: "Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12443-50, 1998 (PubMed).

Validation Images
back to top