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Details for Product No. ABIN649300

Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1) (pSer181) antibody

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Antigen
Synonyms AMPK, HAMPKb, AU021155, E430008F22, 1300015D22Rik, MGC82489
Epitope
»Alternatives pSer181
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 3 references available
Catalog no. ABIN649300
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta was used as immunogen.
Alternative Name AMPK-beta1
Background The 5-AMP-activated protein kinase (AMPK) is a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found, and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phosphorylation on serine 24/25 and serine 182 have been linked to AMPK localization.
Synonyms: PRKAB1, AMPK, 5'-AMP-activated protein kinase subunit beta-1
Molecular Weight 38 kDA
Gene ID 5564
UniProt Q9Y478
Research Area Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:20000 ICC: ~~ 1:100~250 IHC: ~~ 1:100
Comment

Background: The 5-AMP-activated protein kinase (AMPK) is a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found, and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phosphorylation on serine 24/25 and serine 182 have been linked to AMPK localization.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment AMPK-beta1 Antibody Phospho-pS181 can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Davies, Hawley, Woods et al.: "Purification of the AMP-activated protein kinase on ATP-gamma-sepharose and analysis of its subunit structure." in: European journal of biochemistry / FEBS, Vol. 223, Issue 2, pp. 351-7, 1994 (PubMed).

Woods, Cheung, Smith et al.: "Characterization of AMP-activated protein kinase beta and gamma subunits. Assembly of the heterotrimeric complex in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 17, pp. 10282-90, 1996 (PubMed).

Thornton, Snowden, Carling: "Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12443-50, 1998 (PubMed).

Alternatives for antigen "Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1)", type "Antibodies"
Hosts (113), (7)
Reactivities (106), (93), (85), (24), (24), (12)
Applications (90), (65), (34), (30), (24), (7), (4), (2), (2), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (20), (17), (13), (10), (8), (5), (2), (1), (1), (1), (1)
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