Amyloid-Beta A4 (APP) antibody

Antigen: Amyloid-Beta A4 (APP)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649302

Additional Information

Gene ID: 351

UniProt: P05067

Description: Other names: APP, A4, AD1, C31, Gamma-CTF(50),Amyloid intracellular domain 50

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the NPXY motif of human Amyloid Beta A4 precusor (APP) was used as immunogen. This antibody specifically recognizes APP and its isoforms. It will not recognize either of the two Amyloid Beta Protein ( A?4 ) variants, A?1 -40 or A?1 -42.

Molecular Weight: 95 kDA

Comments:

Background: Alzheimer's disease (AD) is characterized by the presence of senile plaques in the brain and blood vessel walls. Amyloid Beta A4 (A?4) protein has been found to be the principal constituent of senile plaques of ADs patients (1-2). The amyloid beta-protein precursor (APP) is proteolytically cleaved to generate the 4 kDa A?4 protein. Two variants of A?4 can be detected, A?1-40 short-tailed and A?1-42 long-tailed (3-4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:20 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Amyloid-Beta A4 Antibody (APP) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Neurology

Restrictions: For Research Use only

Publications

Product: Masters, Simms, Weinman et al.: "Amyloid plaque core protein in Alzheimer disease and Down syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 12, pp. 4245-9, 1985 (PubMed).