AP2 mu1 antibody

Antigen: AP2 mu1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649307

Additional Information

Gene ID: 1173

UniProt: Q96CW1

Description: Other names: AP2M1, CLAPM1, KIAA0109, AP-2 complex subunit mu, Adapter-related protein complex 2 mu subunit,Adaptor protein complex AP-2 subunit mu,Adaptin-mu2,AP-2 mu chain,Plasma membrane adaptor AP-2 50 kDa protein,HA2 50 kDa subunit,Clathrin assembly protein compl

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human AP2 mu1 was used as an immunogen.

Molecular Weight: 50 kDA

Comments:

Background: Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed assembly proteins, or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50 kDa, designated AP50, and a small chain (1). The medium chain AP2 mu1 interacts specifically with the tyrosine-based signals of several integral membrane proteins through the consensus sequence YXXPhi, where X can be any residue and Phi is a large hydrophobic residue (2). Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. It has been shown that the clathrin adaptor complex AP2 is a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~50000 FC: ~~ 1:60 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: AP2 mu1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Druck, Gu, Prabhala et al.: "Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP." in: Genomics, Vol. 30, Issue 1, pp. 94-7, 1996 (PubMed).

Follows, McPheat, Minshull et al.: "Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28." in: The Biochemical journal, Vol. 359, Issue Pt 2, pp. 427-34, 2001 (PubMed).

Krauss, Kukhtina, Pechstein et al.: "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 32, pp. 11934-9, 2006 (PubMed).