Adaptor-Related Protein Complex 2, sigma 1 Subunit (AP2S1) antibody

Antigen: Adaptor-Related Protein Complex 2, sigma 1 Subunit (AP2S1)

Synonyms: AP2S1, MGC140595, ap17, claps2, ap17-delta, DKFZp468J155, AP17, CLAPS2, AP17-DELTA, Ap17, AI043088, MGC62945, MGC85224

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649308

Additional Information

Alternative name: AP2S1

Gene ID: 1175

UniProt: P53680

Description: Other names: AP2S1, AP17, CLAPS2, AP-2 complex subunit sigma, Adapter-related protein complex 2 sigma subunit,Adaptor protein complex AP-2 subunit sigma,Sigma2-adaptin,Plasma membrane adaptor AP-2 17 kDa protein,HA2 17 kDa subunit,Clathrin assembly protein 2 small cha

Characteristics: Rabbit Monoclonal

Specificity: A synthetic peptide corresponding to residues in human AP2S1 was used as an immunogen.

Molecular Weight: 17 kDA

Comments:

Background: Adaptor-related protein complex 2 sigma-1 subunit (AP2S1, AP17) is the component of the adaptor complexes which links clathrin to receptors in coated vesicles. It is the smallest polypeptide chain component of AP-2, a heterotetramer composed of two large adaptins (alpha-type subunit, AP2 alpha, and beta-type subunit, AP2 beta), a medium adaptin (mu-type subunit, AP2 mu1) and a small adaptin (sigma-type subunit, AP2S1). AP-2 is one of two major clathrin-associated protein complexes that interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration (1, 2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:20 FC: ~~ 1:200 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: AP2S1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kirchhausen, Davis, Frucht et al.: "AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast." in: The Journal of biological chemistry, Vol. 266, Issue 17, pp. 11153-7, 1991 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).