Apoptotic Peptidase Activating Factor 1 (APAF1) antibody

Details for Product No. ABIN649309
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Antigen
Reactivity
Human
(193), (102), (82), (2), (2), (2), (2), (1), (1)
Host
Rabbit
(163), (28), (10), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(151), (64), (60), (46), (25), (20), (13), (11), (7), (5), (3), (3), (1)
Pubmed 1 reference available
Quantity 100 μL
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Specificity A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.
Alternative Name Apaf-1 (APAF1 Antibody Abstract)
Background Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.
Synonyms: APAF1, KIAA0413, Apoptotic protease-activating factor 1
Molecular Weight 130 kDA
Gene ID 317
UniProt O14727
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Signaling, Chromatin, Cell Structure
Pathways p53 Pathway
Application Notes IHC: = 1:50-1:100
Comment

Background: Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Apaf-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Saleh, Srinivasula, Acharya et al.: "Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17941-5, 1999 (PubMed).

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Catalog No. ABIN649309
495.71 $
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