APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody

Antigen: APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1)

Synonyms: APE, Apex, HAP1, Ref-1, APEX1, APEN, BAP1, MGC139790, MGC113976, zgc:66204, wu:fc13d11, hm:zehn1532, apex1, MGC76280, MGC79645, MGC78928, APX, APE1, APEX, REF1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649311

Additional Information

Alternative name: Ape-1

Gene ID: 5599

UniProt: P27695

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues near the N-terminus in human Ape 1 was used as an immunogen.

Molecular Weight: 36 kDA

Comments:

Background: Apurinic endonuclease (Ape 1) functions as a major DNA repair enzyme that is essential for embryogenesis and cell viability (1). It is involved in the base excision repair (BER) pathway and acts as a reduction-oxidation signaling factor to maintain transcription factors in an active reduced state (2). Ape 1 plays a critical role as a transcriptional coactivator for different transcription factors (i.e. AP-1, NF-kappaB, p53) in controlling different cellular processes such as apoptosis, proliferation, and differentiation (3). Elevated levels of Ape 1 have been found in cancers such as ovarian, cervical, and prostate (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000~50000 IHC: ~~ 1 : 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Ape-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Evans, Limp-Foster, Kelley: "Going APE over ref-1." in: Mutation research, Vol. 461, Issue 2, pp. 83-108, 2001 (PubMed).

Tell, Damante, Caldwell et al.: "The intracellular localization of APE1/Ref-1: more than a passive phenomenon?" in: Antioxidants & redox signaling, Vol. 7, Issue 3-4, pp. 367-84, 2005 (PubMed).

Fishel, He, Reed et al.: "Knockdown of the DNA repair and redox signaling protein Ape1/Ref-1 blocks ovarian cancer cell and tumor growth." in: DNA repair, Vol. 7, Issue 2, pp. 177-86, 2008 (PubMed).

Manvilla, Varney, Drohat: "Chemical shift assignments for human apurinic/apyrimidinic endonuclease 1." in: Biomolecular NMR assignments, Vol. 4, Issue 1, pp. 5-8, 2010 (PubMed).