|+1 404 474 4654|
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APEX Nuclease (Multifunctional DNA Repair Enzyme) 1 (APEX1) antibody
|Synonyms||APE, Apex, HAP1, Ref-1, APEX1, APEN, BAP1, MGC139790, MGC113976, zgc:66204, wu:fc13d11, hm:zehn1532, apex1, MGC76280, MGC79645, MGC78928, APX, APE1, APEX, REF1|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Rabbit Monoclonal IgG|
|Specificity||A synthetic peptide corresponding to residues near the N-terminus in human Ape 1 was used as an immunogen.|
|Molecular Weight||36 kDA|
Background: Apurinic endonuclease (Ape 1) functions as a major DNA repair enzyme that is essential for embryogenesis and cell viability (1). It is involved in the base excision repair (BER) pathway and acts as a reduction-oxidation signaling factor to maintain transcription factors in an active reduced state (2). Ape 1 plays a critical role as a transcriptional coactivator for different transcription factors (i.e. AP-1, NF-kappaB, p53) in controlling different cellular processes such as apoptosis, proliferation, and differentiation (3). Elevated levels of Ape 1 have been found in cancers such as ovarian, cervical, and prostate (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:10000~50000 IHC: ~~ 1 : 100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Ape-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Evans, Limp-Foster, Kelley: "Going APE over ref-1." in: Mutation research, Vol. 461, Issue 2, pp. 83-108, 2001 (PubMed).
Tell, Damante, Caldwell et al.: "The intracellular localization of APE1/Ref-1: more than a passive phenomenon?" in: Antioxidants & redox signaling, Vol. 7, Issue 3-4, pp. 367-84, 2005 (PubMed).
Fishel, He, Reed et al.: "Knockdown of the DNA repair and redox signaling protein Ape1/Ref-1 blocks ovarian cancer cell and tumor growth." in: DNA repair, Vol. 7, Issue 2, pp. 177-86, 2008 (PubMed).
Manvilla, Varney, Drohat: "Chemical shift assignments for human apurinic/apyrimidinic endonuclease 1." in: Biomolecular NMR assignments, Vol. 4, Issue 1, pp. 5-8, 2010 (PubMed).