Aquaporin 5 (AQP5) antibody

Antigen: Aquaporin 5 (AQP5)

Synonyms: MGC124967, MIP, AQP5, aqp2, LOC100231329

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649321

Additional Information

Alternative name: Aquaporin-5

Gene ID: 9588

UniProt: P55064

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Aquaporin 5 was used as an immunogen.

Molecular Weight: 27 kDA

Comments:

Background: Aquaporin 5 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions (1). It is expressed in epithelia of lung, cornea, and various secretory glands, sites where extracellular osmolality is known to fluctuate (2). Expression of Aquaporin 5 may have pathophysiological implications attributed to a dysfunctional water regulation by Aquaporin 5such as endolymphatic hydrops (i.e. in Meniere's disease) or sensorineural hearing loss (i.e. in Sj�gren's syndrome) (3). Up-regulation of Aquaporin 5 may be involved in differentiation of human gastric cancer cells (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Aquaporin-5 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Da Silva, Silberstein, Beaulieu et al.: "Postnatal expression of aquaporins in epithelial cells of the rat epididymis." in: Biology of reproduction, Vol. 74, Issue 2, pp. 427-38, 2006 (PubMed).

Sidhaye, Güler, Schweitzer et al.: "Transient receptor potential vanilloid 4 regulates aquaporin-5 abundance under hypotonic conditions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 12, pp. 4747-52, 2006 (PubMed).

Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).

Hirt, Penkova, Eckhard et al.: "The subcellular distribution of aquaporin 5 in the cochlea reveals a water shunt at the perilymph-endolymph barrier." in: Neuroscience, Vol. 168, Issue 4, pp. 957-70, 2010 (PubMed).