ADP-Ribosylation Factor 1 (ARF1) antibody

Antigen: ADP-Ribosylation Factor 1 (ARF1)

Synonyms: ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12, arf1, A2MP, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF1GAP, MGC39924, HRIHFB2281, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, Arf1gap, AI115377, Arf GAP1, MGC53624, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, ARF1A1C, T8I13.1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649322

Additional Information

Alternative name: ARF1

Gene ID: 375

UniProt: P84077

Description: Other names: ARF1, ADP-ribosylation factor 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human ARF1 was used as immunogen. This antibody is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.

Molecular Weight: 21 kDA

Comments:

Background: ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase, and it recruits COPI to membranes (4-5).

Synonyms: ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12, arf1, A2MP, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF1GAP, MGC39924, HRIHFB2281, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, Arf1gap, AI115377, Arf GAP1, MGC53624, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, ARF1A1C, T8I13.1

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000 FC: ~~ 1:80 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: ARF1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Serafini, Orci, Amherdt et al.: "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein." in: Cell, Vol. 67, Issue 2, pp. 239-53, 1991 (PubMed).

Bobak, Nightingale, Murtagh et al.: "Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 16, pp. 6101-5, 1989 (PubMed).

Yang, Heimberg, DSouza-Schorey et al.: "Subcellular distribution and differential expression of endogenous ADP-ribosylation factor 6 in mammalian cells." in: The Journal of biological chemistry, Vol. 273, Issue 7, pp. 4006-11, 1998 (PubMed).

Bonifacino, Lippincott-Schwartz: "Coat proteins: shaping membrane transport." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 5, pp. 409-14, 2003 (PubMed).

Nie, Hirsch, Randazzo: "Arf and its many interactors." in: Current opinion in cell biology, Vol. 15, Issue 4, pp. 396-404, 2003 (PubMed).