ADP-Ribosylation Factor 1 (ARF1) antibody

Details for Product No. ABIN649322
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Antigen
Synonyms
ARF-1, ARF1, ARF1_DROME, Arf, Arf1, Arf79f, CG8385, Darf1, Dm Arf79F, Dmel\\CG8385, d-Arf1, dARF79F, dARFI, dArf1, arf-1, MGC52573, TRIM23, arf1, A2MP, MGC53624, arf6, MGC76053, MGC80156, arf1gap, atf ... show more
ARF-1, ARF1, ARF1_DROME, Arf, Arf1, Arf79f, CG8385, Darf1, Dm Arf79F, Dmel\\CG8385, d-Arf1, dARF79F, dARFI, dArf1, arf-1, MGC52573, TRIM23, arf1, A2MP, MGC53624, arf6, MGC76053, MGC80156, arf1gap, atfb1, atfb3, jlns1, k(v)7.1, kcna8, kcna9, kv1.9, kv7.1, kvlqt1, lqt, lqt1, rws, sqt2, wrs, ARFGAP3, ADP-RIBOSYLATION FACTOR, T8I13.1, arf2, wu:fb33e02, wu:fb78h01, ADP-RIBOSYLATION FACTOR 1A, ADP-ribosylation factor 1, ATARF, ATARF1, ATARFA1A, F28C11.12, arf3, si:dz202l16.1, F23H11.7, F23H11_7, auxin response factor 1 show less
Reactivity
Human, Rat (Rattus)
(62), (25), (23), (13), (13), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(51), (15), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(51), (24), (19), (13), (10), (7), (7), (2), (1)
Pubmed 5 references available
Quantity 100 μL
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Catalog No. ABIN649322
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Specificity A synthetic peptide corresponding to residues in human ARF1 was used as immunogen. This antibody is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis.
Alternative Name ARF1
Background ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase, and it recruits COPI to membranes (4-5).
Synonyms: ARF1, ADP-ribosylation factor 1
Molecular Weight 21 kDA
Gene ID 375
UniProt P84077
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure, Signaling, Chromatin
Application Notes IHC: = 1:250-500, WB: = 1:2000
Comment

Background: ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARF family, which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) localizes in the cytoplasm, while the active form (ARF1-GTP) localizes in the membrane (2-3). Active ARF1 is also localized to the Golgi complex by regulating the assembly of coat proteins onto membranes and mediating traffic in the ER-Golgi. ARF1 activates phospholipase D and phosphatidylinositol 4-phosphate 5-kinase, and it recruits COPI to membranes (4-5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment ARF1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Serafini, Orci, Amherdt et al.: "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein." in: Cell, Vol. 67, Issue 2, pp. 239-53, 1991 (PubMed).

Bobak, Nightingale, Murtagh et al.: "Molecular cloning, characterization, and expression of human ADP-ribosylation factors: two guanine nucleotide-dependent activators of cholera toxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 16, pp. 6101-5, 1989 (PubMed).

Yang, Heimberg, DSouza-Schorey et al.: "Subcellular distribution and differential expression of endogenous ADP-ribosylation factor 6 in mammalian cells." in: The Journal of biological chemistry, Vol. 273, Issue 7, pp. 4006-11, 1998 (PubMed).

Bonifacino, Lippincott-Schwartz: "Coat proteins: shaping membrane transport." in: Nature reviews. Molecular cell biology, Vol. 4, Issue 5, pp. 409-14, 2003 (PubMed).

Nie, Hirsch, Randazzo: "Arf and its many interactors." in: Current opinion in cell biology, Vol. 15, Issue 4, pp. 396-404, 2003 (PubMed).

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