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Activating Transcription Factor 2 (ATF2) (pThr71) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Binding Site
Alternatives

pThr71
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN649328
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name ATF-2
Gene ID 1386
UniProt P15336
Description Other names: ATF2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2,cAMP response element-binding protein CRE-BP1,HB16,Cyclic AMP-responsive element-binding protein 2
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF-2 was used as immunogen. The antibody only detects ATF-2 phosphorylated on Threonine 71
Molecular Weight 70 kDA
Comments

Background: ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:40 ICC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage ATF-2 Antibody Phospho (pT71) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Maekawa, Sakura, Kanei-Ishii et al.: "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain." in: The EMBO journal, Vol. 8, Issue 7, pp. 2023-8, 1989 (PubMed).

Livingstone, Patel, Jones: "ATF-2 contains a phosphorylation-dependent transcriptional activation domain." in: The EMBO journal, Vol. 14, Issue 8, pp. 1785-97, 1995 (PubMed).

van Dam, Wilhelm, Herr et al.: "ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents." in: The EMBO journal, Vol. 14, Issue 8, pp. 1798-811, 1995 (PubMed).

Gupta, Campbell, Dérijard et al.: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, 1995 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (267), Mouse (12), Goat (7), Sheep (7)
Reactivities Human (286), Mouse (Murine) (177), Rat (Rattus) (176), Cow (Bovine) (52), Horse (Equine) (44), Dog (Canine) (30), Chicken (22), Pig (Porcine) (20), Rabbit (18), Guinea Pig (5), Bird (Avian) (2), Cat (Feline) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (161), Immunohistochemistry (IHC) (110), Immunofluorescence (IF) (96), ELISA (86), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Immunoprecipitation (IP) (56), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Electrophoretic Mobility-Shift Assay (EMSA) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Functional Studies (Func) (1)
Conjugates Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (8), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes Thr53 (12), pSer472 (8), N-Term (7), pSer322 (7), pSer44 (7), pSer94 (7), pThr51 (6), pThr53 (6), C-Term (5), pThr69, pThr71 (5), pSer498,pSer490 (4), pThr69, pThr51 (4), pSer112, pSer94 (3), pSer44, pSer62 (3), pSer490, pSer498 (3), pSer62, pSer44 (3), pThr51, pThr69 (3), pThr53, pThr71 (3), pThr55, pThr73 (3), pThr71 (3), pThr71, pThr53 (3), pThr73, pThr55 (3), Ab-112 or 94 (2), Ab-62 or 44 (2), Ab-69 or 51 (2), Ab-71 or 53 (2), Ab-73 or 55 (2), Internal Region (2), Ser44 (2), Ser94 (2), pSer112 or 94 (2), pSer62 or 44 (2), pSer94, pSer112 (2), pThr69 or 51 (2), pThr71 or 53 (2), pThr73 or 55 (2), AA 13-27 (1), AA 95-211 (1), Phosphospecific (1), Ser112, Ser94 (1), Ser62 (1), Ser62, Ser44 (1), Ser69, Ser71 (1), Ser94, Ser112 (1), Thr51 (1), Thr71, Thr51 (1), Thr73, Thr55 (1), Thr99, Thr51 (1), pSer112 (1), pSer44,pSer62 (1), pSer480 (1), pSer62 (1), pSer730 (1), pSer90 (1), pSer94, pSer119 (1), pSer94,pSer112 (1), pThr286 (1), pThr34 (1), pThr51,pThr69 (1), pThr53,pThr71 (1), pThr55,pThr73 (1), pThr69 (1), pThr73 (1), pTyr412 (1)