|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Activating Transcription Factor 2 (ATF2) (pThr71) antibody
|Synonyms||HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420|
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|4 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: ATF2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2,cAMP response element-binding protein CRE-BP1,HB16,Cyclic AMP-responsive element-binding protein 2|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF-2 was used as immunogen. The antibody only detects ATF-2 phosphorylated on Threonine 71|
|Molecular Weight||70 kDA|
Background: ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:40 ICC: ~~ 1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||ATF-2 Antibody Phospho (pT71) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Maekawa, Sakura, Kanei-Ishii et al.: "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain." in: The EMBO journal, Vol. 8, Issue 7, pp. 2023-8, 1989 (PubMed).
Livingstone, Patel, Jones: "ATF-2 contains a phosphorylation-dependent transcriptional activation domain." in: The EMBO journal, Vol. 14, Issue 8, pp. 1785-97, 1995 (PubMed).
van Dam, Wilhelm, Herr et al.: "ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents." in: The EMBO journal, Vol. 14, Issue 8, pp. 1798-811, 1995 (PubMed).
Gupta, Campbell, Dérijard et al.: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, 1995 (PubMed).