anti-Aurora Kinase C antibody (AURKC) (N-Term)

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Antigen
Alternatives
Epitope
N-Term
(58), (17), (9), (8), (8), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(115), (21), (19), (1), (1), (1), (1), (1)
Host
Rabbit
(88), (14), (13)
Clonality
Monoclonal
Conjugate
This Aurora Kinase C antibody is un-conjugated
(8), (8), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(98), (57), (30), (19), (11), (8), (7), (3)
Pubmed 2 references available
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Quantity 100 μL
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Specificity A synthetic peptide corresponding to residues near the N-terminus of human Aurora C was used as an immunogen.
Alternative Name Aurora-C (AURKC Antibody Abstract)
Background Aurora-C is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Studies show that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. INCENP binds and activates Aurora-C in vivo and in vitro. Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells (2). Over-expression of an inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone resulting in multinucleation which demonstrates that Aurora C is required for cytokinesis (3).
Synonyms: AURKC, AIE2, AIK3, STK13, Serine/threonine-protein kinase 13, Aurora/Ipl1/Eg2 protein 2,Aurora/Ipl1-related kinase 3,Aurora-C
Molecular Weight 35 kDA
Gene ID 6795
UniProt Q9UQB9
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Pathways Cell Division Cycle
Application Notes The suggested dilution is: WB: = 1:1000
Comment

Background: Aurora-C is a member of a novel family of serine/threonine kinases that have been identified as key regulators of the mitotic cell division process (1). Studies show that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein (INCENP), which are known passenger proteins. INCENP binds and activates Aurora-C in vivo and in vitro. Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells (2). Over-expression of an inactive mutant of Aurora C impaired the localization of Aurora C to the spindle midzone resulting in multinucleation which demonstrates that Aurora C is required for cytokinesis (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aurora-C Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Li, Sakashita, Matsuzaki et al.: "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 47201-11, 2004 (PubMed).

Katayama, Brinkley, Sen: "The Aurora kinases: role in cell transformation and tumorigenesis." in: Cancer metastasis reviews, Vol. 22, Issue 4, pp. 451-64, 2003 (PubMed).