CD86 Molecule (CD86) antibody

Antigen: CD86 Molecule (CD86)

Synonyms: B7, B70, MB7, B7-2, B7.2, CLS1, Ly58, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2, CD86, LOC554318, LAB72, CD28LG2, MGC34413

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649339

Additional Information

Alternative name: CD86

Gene ID: 942

UniProt: P42081

Description: Other names: CD86, CD28LG2, T-lymphocyte activation antigen CD86, Activation B7-2 antigen,CTLA-4 counter-receptor B7.2,B70,FUN-1,BU63

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminus of human B7-2 was used as an immunogen.

Molecular Weight: 70 kDA

Comments:

Background: B7-2 (CD86) is an important costimulatory molecule for the priming and activation of naive and memory T cells, respectively. Soluble CD86 is produced by resting monocytes and results from an alternatively spliced transcript (CD86deltaTM) characterized by deletion of the transmembrane domain (1). Engagement of CD28 with B7-1 and B7-2 ligands on antigen-presenting cells (APCs) provides a stimulatory signal for T-cell activation, whereas subsequent engagement of CTLA-4 with these same ligands results in attenuation of the response. Given their central function in immune modulation, CTLA-4- and CD28-associated signaling pathways are primary therapeutic targets for preventing autoimmune disease, graft versus host disease, graft rejection and promoting tumor immunity (2). It has also been shown that on B cells and macrophages, heat shock proteins GroES and GroEL both stimulated the expression of B7-2 (3)

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000~50000 FC: ~~ 1:10 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: B7-2/CD86 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Jeannin, Magistrelli, Aubry et al.: "Soluble CD86 is a costimulatory molecule for human T lymphocytes." in: Immunity, Vol. 13, Issue 3, pp. 303-12, 2000 (PubMed).

Schwartz, Zhang, Fedorov et al.: "Structural basis for co-stimulation by the human CTLA-4/B7-2 complex." in: Nature, Vol. 410, Issue 6828, pp. 604-8, 2001 (PubMed).