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BCL2-Associated Agonist of Cell Death (BAD) (N-Term) antibody

Antigen

BCL2-Associated Agonist of Cell Death (BAD)
Synonyms BBC2, BCL2L8, wu:fa01b12, wu:fa96d04, MGC72439, BAD, MGC127164
Binding Site
Alternatives

N-Term
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649341
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Bad
Gene ID 572
UniProt Q92934
Description Other names: BAD, BBC6, BCL2L8, Bcl2 antagonist of cell death, Bcl-XL/Bcl-2-associated death promoter
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 members.
Molecular Weight 23 kDA
Comments

Background: Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:10000 IP: ~~ 1:50 FC: ~~ 1:50 ICC: ~~ 1:250~500 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Bad Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Feng, Funk, Wang et al.: "The RNA component of human telomerase." in: Science (New York, N.Y.), Vol. 269, Issue 5228, pp. 1236-41, 1995 (PubMed).

Greider: "Telomere length regulation." in: Annual review of biochemistry, Vol. 65, pp. 337-65, 1996 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "BCL2-Associated Agonist of Cell Death (BAD)", type "Antibodies"
Hosts Rabbit (235), Mouse (14), Chicken (2), Rat (1)
Reactivities Human (209), Mouse (Murine) (135), Rat (Rattus) (120), Horse (Equine) (10), Pig (Porcine) (10), Cow (Bovine) (7), Dog (Canine) (7), Cat (Feline) (1), Chicken (1), Monkey (1)
Applications Western Blotting (WB) (162), ELISA (80), Immunofluorescence (IF) (77), Immunohistochemistry (IHC) (77), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (65), Immunoprecipitation (IP) (28), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Fixed) (IHC (fx)) (3), Cellular Assay (CA) (2), Flow Cytometry (FACS) (2), Immunoassay (IA) (2), Immunoelectron Microscopy (IEM) (2), Immunostaining (ISt) (2), Dot Blot (Dot) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 488 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), PE,Cy5.5 (10), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer112 (18), pSer155 (17), pSer136 (15), C-Term (8), pSer118 (8), pSer134 (8), pSer75 (8), pSer99 (8), Internal Region (6), pSer112,pSer75 (6), pSer128 (4), N-Term (3), Ser112 (3), pSer155,pSer118 (3), BH3 Domain (2), Center (2), Ser136 (2), pSer136,pSer99 (2), AA 100-130 (1), AA 19-35 (1), AA 2-168 (1), AA 2-23 (1), AA 51-61 (1), AA 62-73 (1), AA 87-103 (1), Cleaved-Asp71 (1), Middle Region (1), Phosphospecific (1), Pro102 (1), cleaved (1), pSer123 (1), pSer180 (1), pSer498 (1), pSer775 (1), pSer91 (1), pTyr860,pTyr859 (1), phospho (1)