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BCL2-Associated Athanogene (BAG1) antibody

Antigen

BCL2-Associated Athanogene (BAG1)
Synonyms RAP46
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649342
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Bag-1
UniProt Q99933
Description Other names: BAG1, HAP, BAG family molecular chaperone regulator 1, Bcl-2-associated athanogene 1,Glucocorticoid receptor-associated protein RAP46
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues following the repeat 7 of human Bag-1 was used as immunogen. The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
Molecular Weight 33 / 46 / 50 kDA
Comments

Background: Members of the Bcl-2 family of proteins play varied stimulatory or inhibitory roles in the regulation of programmed cell death, or apoptosis. One member of this family, Bcl2-Interacting Protein-1 (BAG-1), functions in conjunction with Bcl-2 to inhibit apoptosis (1,2). BAG-1 is a heat shock 70 kDa (Hsp70)- binding protein (3) that is localized to either the cytosol, nucleus or both.

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Bag-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Apoptosis/Necrosis, Protein Modifications, Cell Structure
Restrictions For Research Use only

Publications
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Product Takayama, Kochel, Irie et al.: "Cloning of cDNAs encoding the human BAG1 protein and localization of the human BAG1 gene to chromosome 9p12." in: Genomics, Vol. 35, Issue 3, pp. 494-8, 1996 (PubMed).

Matsuzawa, Takayama, Froesch et al.: "p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1." in: The EMBO journal, Vol. 17, Issue 10, pp. 2736-47, 1998 (PubMed).

Takayama, Xie, Reed: "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 781-6, 1999 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "BCL2-Associated Athanogene (BAG1)", type "Antibodies"
Hosts Rabbit (60), Mouse (8)
Reactivities Human (52), Mouse (Murine) (33), Rat (Rattus) (26), Dog (Canine) (20), Horse (Equine) (19), Cow (Bovine) (3), Cat (Feline) (1), Chicken (1), Nematode (1), Pig (Porcine) (1), Rabbit (1)
Applications Western Blotting (WB) (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunofluorescence (IF) (22), ELISA (19), Immunoprecipitation (IP) (19), Immunohistochemistry (IHC) (17), Immunohistochemistry (Fixed) (IHC (fx)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (10), N-Term (10), AA 204-219 (1), AA 205-219 (1), AA 26-45 (1)