BCL2-Associated Athanogene 3 (BAG3) antibody

Details for Product No. ABIN649346
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Antigen
Synonyms BAG-3, BIS, CAIR-1, MFM6, Bis, mg638, AA407278, ATBAG3, BCL-2-associated athanogene 3, T28J14.160, T28J14_160, bag3-A, MGC53113, zgc:100859, BAG3
Reactivity
Human
(44), (26), (26), (13), (13), (12), (12)
Host
Rabbit
(35), (6), (5)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(36), (26), (12), (12), (10), (3), (3), (2), (2)
Pubmed 4 references available
Catalog no. ABIN649346
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in human Bag-3 was used as an immunogen.
Alternative Name Bag-3
Background Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).
Molecular Weight 80 kDA
Gene ID 7531
UniProt O95817
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-10000
Comment

Background: Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Bag-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Homma, Iwasaki, Shelton et al.: "BAG3 deficiency results in fulminant myopathy and early lethality." in: The American journal of pathology, Vol. 169, Issue 3, pp. 761-73, 2006 (PubMed).

Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Virador, Davidson, Czechowicz et al.: "The anti-apoptotic activity of BAG3 is restricted by caspases and the proteasome." in: PLoS ONE, Vol. 4, Issue 4, pp. e5136, 2009 (PubMed).

Hosts (35), (6), (5)
Reactivities (44), (26), (26), (13), (13), (12), (12)
Applications (36), (26), (12), (12), (10), (3), (3), (2), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (6), (3), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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