BCL2-Associated Athanogene 3 (BAG3) antibody

Antigen: BCL2-Associated Athanogene 3 (BAG3)

Synonyms: BIS, BAG-3, CAIR-1, MGC104307, Bis, mg638, AA407278, bag3-A, MGC53113, zgc:100859, BAG3, MGC148719

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649346

Additional Information

Alternative name: Bag-3

Gene ID: 7531

UniProt: O95817

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues in human Bag-3 was used as an immunogen.

Molecular Weight: 80 kDA

Comments:

Background: Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bag-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Homma, Iwasaki, Shelton et al.: "BAG3 deficiency results in fulminant myopathy and early lethality." in: The American journal of pathology, Vol. 169, Issue 3, pp. 761-73, 2006 (PubMed).

Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Virador, Davidson, Czechowicz et al.: "The anti-apoptotic activity of BAG3 is restricted by caspases and the proteasome." in: PLoS ONE, Vol. 4, Issue 4, pp. e5136, 2009 (PubMed).