|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2-Associated Athanogene 3 (BAG3) antibody
|Synonyms||BIS, BAG-3, CAIR-1, MGC104307, Bis, mg638, AA407278, bag3-A, MGC53113, zgc:100859, BAG3, MGC148719|
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Rabbit Monoclonal IgG|
|Specificity||A synthetic peptide corresponding to residues in human Bag-3 was used as an immunogen.|
|Molecular Weight||80 kDA|
Background: Bcl-2-associated athanogene 3 (Bag-3) is a member of a conserved family of cyto-protective proteins that bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone acitivity in a Hip-repressible manner. It has been shown to be prominently expressed in striated muscle and colocalizes with Z-disks. Bag-3 is not required for muscle development, but appears to be critically important for maintenance of mature skeletal muscle (1-2). Bag-3 has also been shown to have anti-apoptotic activity (3). Overexpression of Bag-3 has prevented or reduced the distraction of polyubiquitinated Hsp90/Hsp70 client proteins such as Cyclin D1, Akt, glycogen synthase 3B, and p70s6 kinase, and facilitated cell survival (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Bag-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Homma, Iwasaki, Shelton et al.: "BAG3 deficiency results in fulminant myopathy and early lethality." in: The American journal of pathology, Vol. 169, Issue 3, pp. 761-73, 2006 (PubMed).
Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).
"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).
Virador, Davidson, Czechowicz et al.: "The anti-apoptotic activity of BAG3 is restricted by caspases and the proteasome." in: PLoS ONE, Vol. 4, Issue 4, pp. e5136, 2009 (PubMed).
|Hosts||Rabbit (37), Goat (4), Mouse (1)|
|Reactivities||Human (39), Mouse (Murine) (30), Rat (Rattus) (29), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (24), ELISA (18), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (IHC) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Internal Region (7), C-Term (2), AA 276-294 (1)|