B-Cell CLL/lymphoma 10 (BCL10) antibody

Antigen: B-Cell CLL/lymphoma 10 (BCL10)

Synonyms: CLAP, mE10, CIPER, c-E10, CARMEN, ME10, cE10, BCL-10, C81403, AI132454, BCL10, MGC108338

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649349

Additional Information

Alternative name: Bcl-10

Gene ID: 8915

UniProt: O95999

Description: Other names: BCL10, CIPER, CLAP, B-cell lymphoma/leukemia 10, CARD-like apoptotic protein

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-term of human Bcl-10 was used as immunogen.

Molecular Weight: 32 kDA

Comments:

Background: Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptormediated lymphocyte proliferation and signaling to NF-B, which controls the expression of genes critical for cell survival, proliferation, and immune responses (3-4). Defects in Bcl-10 have been identified in various types of cancer.

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:30 FC: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bcl-10 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure, Cancer, Chromatin

Restrictions: For Research Use only

Publications

Product: Wotherspoon, Pan, Diss et al.: "Cytogenetic study of B-cell lymphoma of mucosa-associated lymphoid tissue." in: Cancer genetics and cytogenetics, Vol. 58, Issue 1, pp. 35-8, 1992 (PubMed).

Ruland, Duncan, Elia et al.: "Bcl10 is a positive regulator of antigen receptor-induced activation of NF-kappaB and neural tube closure." in: Cell, Vol. 104, Issue 1, pp. 33-42, 2001 (PubMed).

Ruefli-Brasse, French, Dixit: "Regulation of NF-kappaB-dependent lymphocyte activation and development by paracaspase." in: Science (New York, N.Y.), Vol. 302, Issue 5650, pp. 1581-4, 2003 (PubMed).