B-Cell CLL/lymphoma 2 (BCL2) antibody

Details for Product No. ABIN649352
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Antigen
Synonyms AW986256, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik, PPP1R50, BCL-2, bcl-2, Bcl2
Reactivity
Human
(454), (160), (126), (54), (36), (36), (14), (12), (3), (2), (2)
Host
Rabbit
(372), (126), (9), (7), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(22), (17), (14), (12), (9), (7), (7), (7), (7), (7), (7), (7), (7), (6), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(339), (181), (140), (123), (90), (87), (70), (58), (55), (43), (24), (10), (5), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN649352
450.00 $
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Specificity A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members
Alternative Name Bcl-2
Background The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).
Molecular Weight 26 kDA
Gene ID 596
UniProt P10415
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes WB: = 1:1000 , FACS: = 1:50 , IHC: = 1:100-1:200
Comment

Background: The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Bcl-2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986 (PubMed).

Deng, Xiao, Lang et al.: "Novel role for JNK as a stress-activated Bcl2 kinase." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23681-8, 2001 (PubMed).

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