BCL2-Like 1 (BCL2L1) antibody

Antigen: BCL2-Like 1 (BCL2L1)

Synonyms: BCLX, BCL2L, Bcl-X, bcl-xL, bcl-xS, BCL-XL/S, DKFZp781P2092, BclX, Bcl2l, bcl-x, Bcl-XL, Bcl-Xs, Bcl(X)L, MGC99998, MGC113803, Bclx, bcl-X, Bcl-xl, BCL2L1, Bcl-xL, BCL-XL, bclx, xR11, bcl2l, bclxl, bcl-XL, bcl-xs, MGC69340, bcl-xl/s, bcl-xl, Bcl-xS

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649354

Additional Information

Alternative name: Bcl-x

Gene ID: 598

UniProt: Q07817

Description: Other names: BCL2L1, BCL2L, BCLX, Bcl-2-like protein 1, Apoptosis regulator Bcl-X

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues between BH3 and BH3 of Human Bcl-x was used as the immunogen. This antibody should recognize Bcl-xL, Bcl-xS and Bcl-x(beta) as the immunogen sequence is common to them. The antibody does not cross-react with other Bcl-2 family members.

Molecular Weight: 26 (Bcl-xL) / 19 (Bcl-xS) kDA

Comments:

Background: Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis (1). Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2 (2). It has been found that eterodimerization with Bax does not seem to be required for anti-apoptotic activity (3). Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival (4). The Bcl-X(S) isoform promotes apoptosis.

Synonyms: BCLX, BCL2L, Bcl-X, bcl-xL, bcl-xS, BCL-XL/S, DKFZp781P2092, BclX, Bcl2l, bcl-x, Bcl-XL, Bcl-Xs, Bcl(X)L, MGC99998, MGC113803, Bclx, bcl-X, Bcl-xl, BCL2L1, Bcl-xL, BCL-XL, bclx, xR11, bcl2l, bclxl, bcl-XL, bcl-xs, MGC69340, bcl-xl/s, bcl-xl, Bcl-xS

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:20 FC: ~~ 1:50 ICC: ~~ 1:100 IHC: ~~ 1:50~1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bcl-x Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Publications

Product: Sedlak, Oltvai, Yang et al.: "Multiple Bcl-2 family members demonstrate selective dimerizations with Bax." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7834-8, 1995 (PubMed).

Cheng, Levine, Boise et al.: "Bax-independent inhibition of apoptosis by Bcl-XL." in: Nature, Vol. 379, Issue 6565, pp. 554-6, 1996 (PubMed).