Breakpoint Cluster Region (BCR) (C-Term) antibody

Antigen: Breakpoint Cluster Region (BCR)

Synonyms: ALL, CML, PHL, BCR1, D22S11, D22S662, FLJ16453, AI561783, AI853148, mKIAA3017, 5133400C09Rik, BCR, zK83D9.7

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649355

Additional Information

Alternative name: Bcr

Gene ID: 613

UniProt: P11274

Description: Other names: BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26

Characteristics: Rabbit Polyclonal IgG Affinity Purified

Specificity: A synthetic peptide corresponding to residues in the C-term of human Bcr was used as immunogen.

Molecular Weight: 130 kDA

Comments:

Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 ICC: ~~ 1:250~500 IHC: ~~ 1:100~250

Purification: Purified

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bcr Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Clark, McLaughlin, Timmons et al.: "Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL)." in: Science (New York, N.Y.), Vol. 239, Issue 4841 Pt 1, pp. 775-7, 1988 (PubMed).

Konopka, Watanabe, Witte: "An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity." in: Cell, Vol. 37, Issue 3, pp. 1035-42, 1984 (PubMed).

Braselmann, McCormick: "Bcr and Raf form a complex in vivo via 14-3-3 proteins." in: The EMBO journal, Vol. 14, Issue 19, pp. 4839-48, 1995 (PubMed).

Boguski, McCormick: "Proteins regulating Ras and its relatives." in: Nature, Vol. 366, Issue 6456, pp. 643-54, 1994 (PubMed).