Breakpoint Cluster Region (BCR) (pTyr177) antibody

Details for Product No. ABIN649357
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Antigen
Synonyms si:dkey-83d9.7, zK83D9.7, BCR, ALL, BCR1, CML, D22S11, D22S662, PHL, 5133400C09Rik, AI561783, AI853148, mKIAA3017
Epitope
pTyr177
(37), (21), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (87), (38), (24), (24), (12), (12), (12), (6), (1)
Host
Rabbit
(110), (15)
Clonality
Monoclonal
Conjugate
Un-conjugated
(5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2)
Application
Western Blotting (WB)
(76), (37), (37), (30), (18), (17), (10), (4), (4), (3), (3), (3)
Pubmed 4 references available
Quantity 100 μL
Options
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Catalog No. ABIN649357
507.14 $
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Specificity A synthetic peptide corresponding to residues surrounding tyrosine 177 of human Bcr was used as immunogen.
Alternative Name Bcr
Background The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).
Synonyms: BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26
Molecular Weight 160 kDA
Gene ID 613
UniProt P11274
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000
Comment

Background: The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML) (1-2). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42 (3). Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Bcr Antibody Phospho (pY177) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Clark, McLaughlin, Timmons et al.: "Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL)." in: Science (New York, N.Y.), Vol. 239, Issue 4841 Pt 1, pp. 775-7, 1988 (PubMed).

Konopka, Watanabe, Witte: "An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity." in: Cell, Vol. 37, Issue 3, pp. 1035-42, 1984 (PubMed).

Braselmann, McCormick: "Bcr and Raf form a complex in vivo via 14-3-3 proteins." in: The EMBO journal, Vol. 14, Issue 19, pp. 4839-48, 1995 (PubMed).

Boguski, McCormick: "Proteins regulating Ras and its relatives." in: Nature, Vol. 366, Issue 6456, pp. 643-54, 1994 (PubMed).

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