Beclin 1, Autophagy Related (BECN1) antibody

Details for Product No. ABIN649358
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Antigen
Synonyms beclin-1, atg6, vps30, beclin1, Beclin-1, becn1, zgc:73097, ATG6, VPS30, 4921513J16Rik, 5430417M23Rik, Atg6
Reactivity
Human
(212), (87), (60), (32), (30), (21), (18), (15), (12), (6), (2), (1), (1)
Host
Rabbit
(162), (56), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (9), (9), (9), (8), (8), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(186), (85), (68), (54), (39), (31), (16), (16), (16), (10), (5), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN649358
450.00 $
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Specificity A synthetic peptide corresponding to residues near the C-terminus of human Beclin-1 was used as an immunogen.
Alternative Name Beclin-1
Background The Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
Synonyms: BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein,Protein GT197
Molecular Weight 52 kDA
Gene ID 8678
UniProt Q14457
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure, Autophagy
Application Notes IHC: = 1:50, WB: = 1:5000
Comment

Background: The Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Beclin-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Liang, Yu, Brown et al.: "Beclin 1 contains a leucine-rich nuclear export signal that is required for its autophagy and tumor suppressor function." in: Cancer research, Vol. 61, Issue 8, pp. 3443-9, 2001 (PubMed).

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