beta-2-Microglobulin (B2M) antibody

Antigen: beta-2-Microglobulin (B2M)

Synonyms: Ly-m11, beta2m, beta2-m, bwm, wu:fa94c05, wu:fb10a09, B2M, LOC398171, b2m-W01, b2m-W03, b2m-Z01, b2m-Z02, b2m-Z03, b2m, B2MG, DKFZp468O042

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649359

Additional Information

Alternative name: Beta-2-Microglobulin

Gene ID: 567

UniProt: P61769

Description: Other names: B2M, Beta-2-microglobulin form pI 5.3

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human beta-2-microglobulin was used as an immunogen.

Molecular Weight: 13 kDA

Comments:

Background: Beta-2-microglobulin (B2M) is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (1). It is a major component forming amyloid deposits in patients with hemodialysis-associated amyloidosis (HAA), a serious complication of long-term hemodialysis (2). B2M is found to contain two antiparallel beta-sheets, each of four beta-strands with the beta-sheets being connected by a single disulfide linkage. No evidence for any regular helical structure is found (3). B2M is an example of a low molecular weight serum protein, with a prominent beta-pleated sheet structure, that may adopt the fibrillar configuration of amyloid in certain pathologic states (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 FC: ~~ 1:60 ICC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Beta-2-Microglobulin Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Okon, Bray, Vucelić: "1H NMR assignments and secondary structure of human beta 2-microglobulin in solution." in: Biochemistry, Vol. 31, Issue 37, pp. 8906-15, 1992 (PubMed).

Güssow, Rein, Ginjaar et al.: "The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 9, pp. 3132-8, 1987 (PubMed).

Gorevic, Casey, Stone et al.: "Beta-2 microglobulin is an amyloidogenic protein in man." in: The Journal of clinical investigation, Vol. 76, Issue 6, pp. 2425-9, 1986 (PubMed).

Miyata, Inagi, Wada et al.: "Glycation of human beta 2-microglobulin in patients with hemodialysis-associated amyloidosis: identification of the glycated sites." in: Biochemistry, Vol. 33, Issue 40, pp. 12215-21, 1994 (PubMed).