anti-beta Actin antibody (Actin, beta)

Details for Product anti-ACTB Antibody No. ABIN649360
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Human, Rat (Rattus)
(486), (209), (188), (52), (48), (42), (38), (35), (28), (25), (22), (15), (13), (12), (11), (10), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(301), (199), (22), (1), (1)
Clonality
Monoclonal
Conjugate
This beta Actin antibody is un-conjugated
(29), (21), (14), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(461), (216), (141), (94), (92), (55), (47), (28), (20), (11), (11), (9), (9), (8), (3), (3), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μL
Options
Shipping to United States ( )
Availability Please contact our customer support.
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Specificity A synthetic peptide corresponding to residues near the C-terminus of human Beta Actin (Cytoplasmic Actin) was used as an immunogen.
Alternative Name Beta-Actin (ACTB Antibody Abstract)
Background Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Inhibition of glycolysis with 2-deoxyglucose induced a rapid transformation of Beta-actin-rich pseudopodia and prevented cell motility, therefore a localized glycolytic supply of energy regulates the formation of Beta-actin-rich pseudopodial protrusions and thereby the motility of invasive tumor cells (2). Beta-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of Beta -Actin is generally used as an internal control for experimental error.
Synonyms: ACTB, Actin, cytoplasmic 1, N-terminally processed, Beta-actin
Molecular Weight 42-43 kDA
Gene ID 60
UniProt P60709
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes IHC: = 1:50, WB: = 1:1000-5000
Comment

Background: Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Inhibition of glycolysis with 2-deoxyglucose induced a rapid transformation of β,-actin-rich pseudopodia and prevented cell motility, therefore a localized glycolytic supply of energy regulates the formation of β,-actin-rich pseudopodial protrusions and thereby the motility of invasive tumor cells (2). β,-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β, -Actin is generally used as an internal control for experimental error.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Beta-Actin Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Nguyen, Wang, Zalzal et al.: "Purification and characterization of beta-actin-rich tumor cell pseudopodia: role of glycolysis." in: Experimental cell research, Vol. 258, Issue 1, pp. 171-83, 2000 (PubMed).

Herman: "Actin isoforms." in: Current opinion in cell biology, Vol. 5, Issue 1, pp. 48-55, 1993 (PubMed).

Validation Images
Catalog No. ABIN649360
450.00 $
Plus shipping costs $45.00
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?