Arrestin, beta 1 (ARRB1) antibody

Antigen: Arrestin, beta 1 (ARRB1)

Synonyms: ARB1, ARR1, BARRES, AW208571, MGC124269, MGC124270, 1200006I17Rik, G430100A01Rik, arrb1, MGC83787, arb1, arr1, barr1, MGC81940, betaarr1, ARRB1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649362

Additional Information

Alternative name: Beta-Arrestin-1

Gene ID: 408

UniProt: P49407

Description: Other names: ARRB1, ARR1, Beta-arrestin-1, Arrestin beta-1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near N-terminus of human -arrestin-1 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.

Molecular Weight: 50 kDA

Comments:

Background: Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). -arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for -arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~20000 FC: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Beta-Arrestin-1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Transcription Factors, Signaling

Restrictions: For Research Use only

Publications

Product: Lefkowitz: "G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18677-80, 1998 (PubMed).

Lin, Daaka, Lefkowitz: "beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor." in: The Journal of biological chemistry, Vol. 273, Issue 48, pp. 31640-3, 1998 (PubMed).

Lin, Miller, Luttrell et al.: "Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 15971-4, 1999 (PubMed).

Miller, Lefkowitz: "Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking." in: Current opinion in cell biology, Vol. 13, Issue 2, pp. 139-45, 2001 (PubMed).