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Details for Product No. ABIN649363

Arrestin, beta 1 (ARRB1) (pSer412) antibody

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Antigen
Synonyms ARB1, ARR1, BARRES, 1200006I17Rik, AW208571, G430100A01Rik, arrb1, MGC83787, arb1, arr1, barr1, betaarr1
Epitope
»Alternatives pSer412
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN649363
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human Beta Arrestin-1 was used as immunogen. The antibody only detects Beta Arrestin-1 phosphorylated on Serine 412.
Alternative Name Beta-Arrestin-1
Background Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). Beta-arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for Beta-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).
Synonyms: ARRB1, ARR1, Beta-arrestin-1, Arrestin beta-1
Molecular Weight 50 kDA
Gene ID 408
UniProt P49407
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Transcription Factors, Signaling
Application Notes The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:150 ICC: ~~ 1:100~1:250 IHC: ~~ 1:500
Comment

Background: Beta-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). Beta-arrestin-1 is phosphorylated by Erk1/2 at Serine 412 of the C-terminus (2). Expanded roles for Beta-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Beta-Arrestin-1 Antibody Phospho (pS412) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Lefkowitz: "G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization." in: The Journal of biological chemistry, Vol. 273, Issue 30, pp. 18677-80, 1998 (PubMed).

Lin, Daaka, Lefkowitz: "beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor." in: The Journal of biological chemistry, Vol. 273, Issue 48, pp. 31640-3, 1998 (PubMed).

Lin, Miller, Luttrell et al.: "Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 15971-4, 1999 (PubMed).

Miller, Lefkowitz: "Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking." in: Current opinion in cell biology, Vol. 13, Issue 2, pp. 139-45, 2001 (PubMed).

Alternatives for antigen "Arrestin, beta 1 (ARRB1)", type "Antibodies"
Hosts (62), (8), (1)
Reactivities (69), (46), (45), (25), (12), (12), (12), (12), (1)
Applications (51), (26), (20), (14), (12), (11), (6), (5), (3), (2), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (18), (11), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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