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Details for Product No. ABIN649369

Dystroglycan 1 (DAG1) antibody

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Antigen
Synonyms DG, Dp71, Dp427, D9Wsu13e
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN649369
Quantity 100 µL
Price
392.86 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding o residues near the C-terminus of human Beta-Dystroglycan was used as an immunogen.
Alternative Name Beta-Dystroglycan
Background The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).
Synonyms: DAG1, Beta-dystroglycan, Dystrophin-associated glycoprotein 1
Molecular Weight 43 kDA
Gene ID 1605
UniProt Q14118
Research Area Cell Signaling, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000
Comment

Background: The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Beta-Dystroglycan Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Ibraghimov-Beskrovnaya, Milatovich, Ozcelik et al.: "Human dystroglycan: skeletal muscle cDNA, genomic structure, origin of tissue specific isoforms and chromosomal localization." in: Human molecular genetics, Vol. 2, Issue 10, pp. 1651-7, 1994 (PubMed).

Yamada, Saito, Fukuta-Ohi et al.: "Processing of beta-dystroglycan by matrix metalloproteinase disrupts the link between the extracellular matrix and cell membrane via the dystroglycan complex." in: Human molecular genetics, Vol. 10, Issue 15, pp. 1563-9, 2001 (PubMed).

Spence, Chen, Batchelor et al.: "Ezrin-dependent regulation of the actin cytoskeleton by beta-dystroglycan." in: Human molecular genetics, Vol. 13, Issue 15, pp. 1657-68, 2004 (PubMed).

Alternatives for antigen "Dystroglycan 1 (DAG1)", type "Antibodies"
Hosts (4)
Reactivities (4), (4), (4), (4), (4), (4)
Applications (4)
Conjugates (1), (1), (1), (1)
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