BH3 Interacting Domain Death Agonist (BID) antibody

Antigen: BH3 Interacting Domain Death Agonist (BID)

Synonyms: FP497, MGC15319, MGC42355, AI875481, AU022477, 2700049M22Rik, BID, MGC140420, bid, si:ch211-238n5.6, xbid, fp497, DKFZp469E066

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649375

Additional Information

Alternative name: Bid

Gene ID: 637

UniProt: P55957

Description: Other names: BID, BH3-interacting domain death agonist p11, p11 BID

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues before the BH3 domain of human Bid was used as the immunogen. This antibody does not cross-react with other Bcl-2 family members.

Molecular Weight: 22 kDA

Comments:

Background: Bid, BH3 interacting domain death agonist, is a pro-apoptotic member of the Bcl-2 family of proteins that regulate apotosis. Bid is localized to the cytosol in an inactive precursor form. Upon caspase-8 binding in the Fas signaling pathway, Bid is cleaved and the active Bid fragment translocates to mitochondria and induces cytochrome c release and the resulting apoptotic cascade (1-3). Bid contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:10 FC: ~~ 1:50 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bid Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Luo, Budihardjo, Zou et al.: "Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors." in: Cell, Vol. 94, Issue 4, pp. 481-90, 1998 (PubMed).

Gross, Yin, Wang et al.: "Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 1156-63, 1999 (PubMed).