BH3 Interacting Domain Death Agonist (BID) antibody

Details for Product No. ABIN649375
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Antigen
Synonyms FP497, AI875481, AU022477, 2700049M22Rik, BID, bid, si:ch211-238n5.6, fp497, xbid, DKFZp469E066
Reactivity
Human
(159), (106), (54), (1), (1)
Host
Rabbit
(176), (42), (8), (5)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(180), (113), (61), (53), (40), (28), (18), (15), (12), (9), (3), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN649375
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues before the BH3 domain of human Bid was used as the immunogen. This antibody does not cross-react with other Bcl-2 family members.
Alternative Name Bid
Background Bid, BH3 interacting domain death agonist, is a pro-apoptotic member of the Bcl-2 family of proteins that regulate apotosis. Bid is localized to the cytosol in an inactive precursor form. Upon caspase-8 binding in the Fas signaling pathway, Bid is cleaved and the active Bid fragment translocates to mitochondria and induces cytochrome c release and the resulting apoptotic cascade (1-3). Bid contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax (4).
Synonyms: BID, BH3-interacting domain death agonist p11, p11 BID
Molecular Weight 22 kDA
Gene ID 637
UniProt P55957
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:100
Comment

Background: Bid, BH3 interacting domain death agonist, is a pro-apoptotic member of the Bcl-2 family of proteins that regulate apotosis. Bid is localized to the cytosol in an inactive precursor form. Upon caspase-8 binding in the Fas signaling pathway, Bid is cleaved and the active Bid fragment translocates to mitochondria and induces cytochrome c release and the resulting apoptotic cascade (1-3). Bid contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Bid Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Luo, Budihardjo, Zou et al.: "Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors." in: Cell, Vol. 94, Issue 4, pp. 481-90, 1998 (PubMed).

Gross, Yin, Wang et al.: "Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not tumor necrosis factor-R1/Fas death." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 1156-63, 1999 (PubMed).

Hosts (176), (42), (8), (5)
Reactivities (159), (106), (54), (1), (1)
Applications (180), (113), (61), (53), (40), (28), (18), (15), (12), (9), (3), (2), (2), (2), (2), (1), (1)
Conjugates (10), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2)
Epitopes (24), (18), (17), (14), (12), (7), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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