BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody

Antigen: BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11)

Synonyms: BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649376

Additional Information

Alternative name: Bim

Gene ID: 10018

UniProt: O43521

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the exons 2 of human Bim was used as immunogen. The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.

Molecular Weight: 22 kDA

Comments:

Background: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members (1). Bim promotes apoptosis by binding to and inhibiting the activity of anti-apoptotic Bcl-2 family members, inducing the release of cytochrome c from mitochondria (2). Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Bim Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: OConnor, Strasser, OReilly et al.: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998 (PubMed).

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998 (PubMed).

Puthalakath, Huang, OReilly et al.: "The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex." in: Molecular cell, Vol. 3, Issue 3, pp. 287-96, 1999 (PubMed).