|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) antibody
|Synonyms||BAM, BIM, BOD, BimL, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6, Bim, Bod, 1500006F24Rik, bim|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in the exons 2 of human Bim was used as immunogen. The antibody can recognize all isoforms of Bim. The antibody does not cross-react with other Bcl-2 family members.|
|Molecular Weight||22 kDA|
Background: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members (1). Bim promotes apoptosis by binding to and inhibiting the activity of anti-apoptotic Bcl-2 family members, inducing the release of cytochrome c from mitochondria (2). Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis (3,4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:40 ICC: ~~ 1:100 IHC: ~~ 1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Bim Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
OConnor, Strasser, OReilly et al.: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998 (PubMed).
Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998 (PubMed).
Puthalakath, Huang, OReilly et al.: "The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex." in: Molecular cell, Vol. 3, Issue 3, pp. 287-96, 1999 (PubMed).