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CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) (C-Term) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||C/EBP alpha|
|Description||Other names: CEBPA, CCAAT/enhancer-binding protein alpha|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in the C-term of human C/EBP alpha was used as immunogen.|
|Molecular Weight||43 kDA|
Background: CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (α,,β,,γ,,δ,,ε,, ζ, ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor (5).
|Application Notes||The suggested dilution is: WB: ~~ 1: 500 IP: ~~ 1:50 FC: ~~ 1:30|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||C/EBP alpha Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
Miller, De Vos, Guerre-Millo et al.: "The adipocyte specific transcription factor C/EBPalpha modulates human ob gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 11, pp. 5507-11, 1996 (PubMed).
Timchenko, Wilde, Nakanishi et al.: "CCAAT/enhancer-binding protein alpha (C/EBP alpha) inhibits cell proliferation through the p21 (WAF-1/CIP-1/SDI-1) protein." in: Genes & development, Vol. 10, Issue 7, pp. 804-15, 1996 (PubMed).
Wang, Iakova, Wilde et al.: "C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4." in: Molecular cell, Vol. 8, Issue 4, pp. 817-28, 2001 (PubMed).