CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) (N-Term) antibody

Antigen: CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA)

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649390

Additional Information

Alternative name: C/EBP alpha

Gene ID: 1050

UniProt: P49715

Description: Other names: CEBPA, CCAAT/enhancer-binding protein alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the N-term of human C/EBP alpha was used as immunogen. This antibody recognizes both the 42kDa and the 30kDa isoforms.

Molecular Weight: 43/30 kDA

Comments:

Background: CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (α,,β,,γ,,δ,,ε,, ζ, ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 FC: ~~ 1:30 ICC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: C/EBP alpha Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Miller, De Vos, Guerre-Millo et al.: "The adipocyte specific transcription factor C/EBPalpha modulates human ob gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 11, pp. 5507-11, 1996 (PubMed).

Timchenko, Wilde, Nakanishi et al.: "CCAAT/enhancer-binding protein alpha (C/EBP alpha) inhibits cell proliferation through the p21 (WAF-1/CIP-1/SDI-1) protein." in: Genes & development, Vol. 10, Issue 7, pp. 804-15, 1996 (PubMed).

Wang, Iakova, Wilde et al.: "C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4." in: Molecular cell, Vol. 8, Issue 4, pp. 817-28, 2001 (PubMed).