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CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB) (C-Term) antibody
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||C/EBP Beta|
|Description||Other names: Cebpb, Crp2, Sfb, CCAAT/enhancer-binding protein beta, C/EBP-related protein 2|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in C-terminus of rat C/EBP b was used as immunogen. The antibody can recognize three C/EBPb isoform (i.e. LAP*, LAP, and LIP).|
|Molecular Weight||38/35/20 kDA|
Background: CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (α,,β,,γ,,δ,,ε,, ζ, ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP-β,, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP β, occurs predominantly as a heterodimer (3), C/EBP β, & γ, readily heterodimerize with each other as well as with C/EBP α, (4). Additionally, 3 isoforms of C /EBP β, can be detected (LAP*, LAP, & LIP) (5).
|Synonyms||LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080, C/EBP-beta, NF-M, Nfil6, IL-6DBP, C/EBPbeta, Il6dbp, CEBPB, C/EBP beta, lap, crp2, tcf5, il6dbp, nf-il6, MGC107842, c/ebp-beta, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, LOC100136165|
|Application Notes||The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:30 ICC: ~~ 1:50~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||C/EBP Beta Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Thomassin, Hamel, Bernier et al.: "Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the alpha 1-fetoprotein gene. Further analysis of C/EBP beta and C/EBP gamma." in: Nucleic acids research, Vol. 20, Issue 12, pp. 3091-8, 1992 (PubMed).
Lekstrom-Himes, Xanthopoulos: "Biological role of the CCAAT/enhancer-binding protein family of transcription factors." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 28545-8, 1998 (PubMed).
Parkin, Baer, Copeland et al.: "Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP)." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23563-72, 2002 (PubMed).
|Hosts||Rabbit (50), Mouse (16)|
|Reactivities||Human (58), Mouse (Murine) (39), Rat (Rattus) (37), Cow (Bovine) (25), Pig (Porcine) (25), Chicken (18), Sheep (Ovine) (18), Dog (Canine) (5)|
|Applications||Western Blotting (WB) (40), Immunofluorescence (IF) (28), ELISA (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (6), Flow Cytometry (FACS) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)|
|Conjugates||Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer105 (6), pThr235 (4), C-Term (3), N-Term (3), AA 1-271 (2), AA 39-78 (1), Internal Region (1), pThr226,pThr222 (1), pThr235, pThr188 (1)|