CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB) (C-Term) antibody

Antigen: CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB)

Synonyms: LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080, C/EBP-beta, NF-M, Nfil6, IL-6DBP, C/EBPbeta, Il6dbp, CEBPB, C/EBP beta, lap, crp2, tcf5, il6dbp, nf-il6, MGC107842, c/ebp-beta, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, LOC100136165

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649391

Additional Information

Alternative name: C/EBP Beta

Gene ID: 24253

UniProt: P21272

Description: Other names: Cebpb, Crp2, Sfb, CCAAT/enhancer-binding protein beta, C/EBP-related protein 2

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in C-terminus of rat C/EBP b was used as immunogen. The antibody can recognize three C/EBPb isoform (i.e. LAP*, LAP, and LIP).

Molecular Weight: 38/35/20 kDA

Comments:

Background: CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (α,,β,,γ,,δ,,ε,, ζ, ) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP-β,, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP β, occurs predominantly as a heterodimer (3), C/EBP β, & γ, readily heterodimerize with each other as well as with C/EBP α, (4). Additionally, 3 isoforms of C /EBP β, can be detected (LAP*, LAP, & LIP) (5).

Synonyms: LAP, CRP2, TCF5, IL6DBP, NF-IL6, MGC32080, C/EBP-beta, NF-M, Nfil6, IL-6DBP, C/EBPbeta, Il6dbp, CEBPB, C/EBP beta, lap, crp2, tcf5, il6dbp, nf-il6, MGC107842, c/ebp-beta, xC/EBPbeta, cebpbb, cb380, c/ebpb, fe11e05, wu:fe11e05, LOC100136165

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:30 ICC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: C/EBP Beta Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Thomassin, Hamel, Bernier et al.: "Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the alpha 1-fetoprotein gene. Further analysis of C/EBP beta and C/EBP gamma." in: Nucleic acids research, Vol. 20, Issue 12, pp. 3091-8, 1992 (PubMed).

Lekstrom-Himes, Xanthopoulos: "Biological role of the CCAAT/enhancer-binding protein family of transcription factors." in: The Journal of biological chemistry, Vol. 273, Issue 44, pp. 28545-8, 1998 (PubMed).

Parkin, Baer, Copeland et al.: "Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP)." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23563-72, 2002 (PubMed).