Caldesmon 1 (CALD1) antibody

Details for Product No. ABIN649405
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, l-Cad, 4833423D12Rik, AI195384, AV071549, AW536160, C920027I18Rik, CALD1, caldesmon, zgc:56389, si:bz30i22.4, si:rp71-30i22.4
Reactivity
Human, Rat (Rattus)
(120), (68), (64), (26), (25), (18), (9), (4), (3), (3), (1), (1), (1), (1), (1)
Host
Rabbit
(97), (37)
Clonality
Monoclonal
Conjugate
Un-conjugated
(5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(102), (53), (36), (35), (32), (20), (19), (7), (4), (4), (4), (4), (3)
Pubmed 3 references available
Catalog no. ABIN649405
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen.
Alternative Name Caldesmon (CaM)
Background Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
Synonyms: CALD1, CAD, CDM, Caldesmon
Molecular Weight 120-150 (smooth muscle)/70-80 (non-muscle) kDA
Gene ID 800
UniProt Q05682
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-2000 , IHC: = 1:250
Comment

Background: Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Caldesmon Antibody (CaM) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Humphrey, Herrera-Sosa, Gonzalez et al.: "Cloning of cDNAs encoding human caldesmons." in: Gene, Vol. 112, Issue 2, pp. 197-204, 1992 (PubMed).

Bryan, Imai, Lee et al.: "Cloning and expression of a smooth muscle caldesmon." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13873-9, 1989 (PubMed).

Fraser, Copeland, Bing et al.: "The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4." in: Biochemistry, Vol. 36, Issue 18, pp. 5483-92, 1997 (PubMed).

Hosts (97), (37)
Reactivities (120), (68), (64), (26), (25), (18), (9), (4), (3), (3), (1), (1), (1), (1), (1)
Applications (102), (53), (36), (35), (32), (20), (19), (7), (4), (4), (4), (4), (3)
Conjugates (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (20), (10), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top