Caldesmon 1 (CALD1) antibody

Details for Product No. ABIN649405
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Antigen
Synonyms CALD1, caldesmon, si:bz30i22.4, si:rp71-30i22.4, zgc:56389, CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, 4833423D12Rik, AI195384, AV071549, AW536160, C920027I18Rik, l-Cad
Reactivity
Human, Rat (Rattus)
(130), (71), (64), (26), (25), (16), (6), (3), (2), (2), (1), (1), (1)
Host
Rabbit
(104), (37)
Clonality
Monoclonal
Conjugate
Un-conjugated
(5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(104), (51), (38), (34), (33), (20), (15), (10), (5), (4), (4), (4), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN649405
450.00 $
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Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen.
Alternative Name Caldesmon (CaM)
Background Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
Synonyms: CALD1, CAD, CDM, Caldesmon
Molecular Weight 120-150 (smooth muscle)/70-80 (non-muscle) kDA
Gene ID 800
UniProt Q05682
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-2000 , IHC: = 1:250
Comment

Background: Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caldesmon Antibody (CaM) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Humphrey, Herrera-Sosa, Gonzalez et al.: "Cloning of cDNAs encoding human caldesmons." in: Gene, Vol. 112, Issue 2, pp. 197-204, 1992 (PubMed).

Bryan, Imai, Lee et al.: "Cloning and expression of a smooth muscle caldesmon." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13873-9, 1989 (PubMed).

Fraser, Copeland, Bing et al.: "The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4." in: Biochemistry, Vol. 36, Issue 18, pp. 5483-92, 1997 (PubMed).

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