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Details for Product No. ABIN649406

Caldesmon 1 (CALD1) antibody

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Antigen
Synonyms CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, l-Cad, 4833423D12Rik, AI195384, AV071549, AW536160, C920027I18Rik, CALD1, caldesmon, zgc:56389, si:bz30i22.4, si:rp71-30i22.4
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN649406
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues near the N-term of human Caldesmon was used as immunogen.
Alternative Name Caldesmon (CaM)
Background Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
Synonyms: CALD1, CAD, CDM, Caldesmon
Molecular Weight 120-150 (smooth muscle)/70-80 (non-muscle) kDA
Gene ID 800
UniProt Q05682
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:5000~20000 IP: ~~ 1:50 ICC: ~~ 1:250~500 IHC: ~~ 1:250~500
Comment

Background: Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Caldesmon Antibody (CaM) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Humphrey, Herrera-Sosa, Gonzalez et al.: "Cloning of cDNAs encoding human caldesmons." in: Gene, Vol. 112, Issue 2, pp. 197-204, 1992 (PubMed).

Bryan: "Caldesmon: fragments, sequence, and domain mapping." in: Annals of the New York Academy of Sciences, Vol. 599, pp. 100-10, 1990 (PubMed).

Bryan, Imai, Lee et al.: "Cloning and expression of a smooth muscle caldesmon." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13873-9, 1989 (PubMed).

Fraser, Copeland, Bing et al.: "The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4." in: Biochemistry, Vol. 36, Issue 18, pp. 5483-92, 1997 (PubMed).

Alternatives for antigen "Caldesmon 1 (CALD1)", type "Antibodies"
Hosts (87), (44)
Reactivities (114), (64), (62), (26), (25), (21), (11), (6), (6), (4), (3), (2), (1), (1), (1)
Applications (99), (53), (35), (35), (29), (20), (17), (10), (6), (6), (5), (3), (2)
Conjugates (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (17), (13), (5), (2), (2), (2), (2), (1), (1), (1), (1)
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