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Details for Product No. ABIN649408

Caldesmon 1 (CALD1) (pThr730) antibody

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Antigen
Synonyms CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, l-Cad, 4833423D12Rik, AI195384, AV071549, AW536160, C920027I18Rik, CALD1, caldesmon, zgc:56389, si:bz30i22.4, si:rp71-30i22.4
Epitope
»Alternatives pThr730
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunoprecipitation (IP)
Pubmed 4 references available
Catalog no. ABIN649408
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A phospho-specific peptide corresponding to residues surrounding Threonine 730 of human Caldesmon was used as an immunogen. The antibody only detects Caldesmon phosphorylated on Threonine 730.
Alternative Name Caldesmon
Background Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).
Synonyms: CALD1, CAD, CDM, Caldesmon
Molecular Weight 70-80 kDA
Gene ID 800
UniProt Q05682
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:10
Comment

Background: Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Caldesmon Antibody Phospho (pT730) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Humphrey, Herrera-Sosa, Gonzalez et al.: "Cloning of cDNAs encoding human caldesmons." in: Gene, Vol. 112, Issue 2, pp. 197-204, 1992 (PubMed).

Bryan: "Caldesmon: fragments, sequence, and domain mapping." in: Annals of the New York Academy of Sciences, Vol. 599, pp. 100-10, 1990 (PubMed).

Bryan, Imai, Lee et al.: "Cloning and expression of a smooth muscle caldesmon." in: The Journal of biological chemistry, Vol. 264, Issue 23, pp. 13873-9, 1989 (PubMed).

Fraser, Copeland, Bing et al.: "The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4." in: Biochemistry, Vol. 36, Issue 18, pp. 5483-92, 1997 (PubMed).

Alternatives for antigen "Caldesmon 1 (CALD1)", type "Antibodies"
Hosts (87), (44)
Reactivities (114), (64), (62), (26), (25), (21), (11), (6), (6), (4), (3), (2), (1), (1), (1)
Applications (99), (53), (35), (35), (30), (20), (18), (10), (6), (6), (5), (3), (2)
Conjugates (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (17), (13), (5), (2), (2), (2), (2), (1), (1), (1)
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