Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) antibody

Details for Product No. ABIN649430
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Antigen
Synonyms CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57, ICH-1, Nedd2, Caspase-2, ICH-1L/1S, ICH1L1S, xCaspase-2
Reactivity
Human
(133), (51), (35), (12), (12), (6), (5), (1)
Host
Rabbit
(111), (29), (8)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(120), (67), (29), (23), (17), (14), (10), (8), (7), (6), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN649430
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to C-terminus of human Caspase-2 p18 subunit was used as immunogen. The antibody should recognize both pro-form (~50kDa) and p18 cleaved-form of Caspase-2. Predicted to detect the splice isoform (35 kDa) based on sequence homology.
Alternative Name Caspase-2
Background Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase, in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).
Synonyms: CASP2, ICH1, NEDD2, Caspase-2 subunit p12, ICH-1 protease,Neural precursor cell expressed developmentally down-regulated protein 2
Molecular Weight 48/18 kDA
Gene ID 835
UniProt P42575
Research Area Apoptosis/Necrosis, Signaling, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500
Comment

Background: Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase, in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Wang, Miura, Bergeron et al.: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994 (PubMed).

Li, Bergeron, Cryns et al.: "Activation of caspase-2 in apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 34, pp. 21010-7, 1997 (PubMed).

Paroni, Henderson, Schneider et al.: "Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21907-15, 2001 (PubMed).

Dirsch, Kirschke, Estermeier et al.: "Apoptosis signaling triggered by the marine alkaloid ascididemin is routed via caspase-2 and JNK to mitochondria." in: Oncogene, Vol. 23, Issue 8, pp. 1586-93, 2004 (PubMed).

Hosts (111), (29), (8)
Reactivities (133), (51), (35), (12), (12), (6), (5), (1)
Applications (120), (67), (29), (23), (17), (14), (10), (8), (7), (6), (2), (1), (1)
Conjugates (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (10), (8), (7), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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