Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) antibody

Details for Product No. ABIN649431
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Antigen
Synonyms
xcpp32, Lice, CPP32, SCA-1, CPP32B, CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRI ... show more
xcpp32, Lice, CPP32, SCA-1, CPP32B, CC3, CG14902, Cas3, Casp 3, Casp3, Caspase-3, DECAY, Decay, Dmel\\CG14902, Drosophila executioner caspase related to Apopain/Yama, caspase 3, caspase-3, CG7788, DRICE, Dmel\\CG7788, DrICE, DrIce, Drice, ICE, crice, drICE, drIce, drice, ice show less
Reactivity
Human, Rat (Rattus)
(279), (104), (89), (20), (18), (17), (5), (5), (4), (4), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(249), (54), (7), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(10), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(253), (95), (94), (90), (52), (22), (15), (15), (14), (12), (10), (4), (4), (2), (2), (2), (1)
Pubmed 3 references available
Catalog no. ABIN649431
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Specificity A synthetic peptide corresponding to residues in p17 subunit of human Caspase-3 was used as immunogen. The antibody should recognize both pro-form (35kDa) and p17 cleaved-form of Caspase-3.
Alternative Name Caspase-3
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Synonyms: CASP3, CPP32, Caspase-3 subunit p12, Yama protein,SREBP cleavage activity 1
Molecular Weight 35 kDA
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1: 5000-50000 , IHC: = 1:25-1:50
Comment

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Caspase-3 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Nicholson, Ali, Thornberry et al.: "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis." in: Nature, Vol. 376, Issue 6535, pp. 37-43, 1995 (PubMed).

Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).

Hosts (249), (54), (7), (2)
Reactivities (279), (104), (89), (20), (18), (17), (5), (5), (4), (4), (4), (3), (2), (2), (2), (1)
Applications (253), (95), (94), (90), (52), (22), (15), (15), (14), (12), (10), (4), (4), (2), (2), (2), (1)
Conjugates (10), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (24), (20), (14), (13), (12), (11), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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