anti-Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (pro-form)

Details for Product anti-CASP3 Antibody No. ABIN649434
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Antigen
Epitope
pro-form
(40), (31), (31), (25), (24), (18), (15), (15), (14), (13), (12), (12), (12), (8), (8), (7), (6), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(466), (179), (137), (21), (12), (10), (9), (9), (8), (7), (6), (5), (4), (4), (3), (2), (1)
Host
Rabbit
(449), (79), (18), (2), (2)
Clonality
Monoclonal
Conjugate
This Caspase 3 antibody is un-conjugated
(25), (22), (18), (11), (11), (11), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(413), (210), (178), (110), (72), (55), (33), (28), (27), (21), (21), (20), (19), (6), (4), (2), (2), (2), (2), (2)
Pubmed 3 references available
Quantity 100 μL
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Specificity A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.
Alternative Name Caspase-3 (CASP3 Antibody Abstract)
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Synonyms: CASP3, CPP32, Caspase-3 subunit p12, Yama protein,SREBP cleavage activity 1
Molecular Weight 35 kDA
Gene ID 836
UniProt P42574
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes IHC: = 1:250, WB: = 1:50000-100000
Comment

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-3 Antibody (Pro) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).

Tewari, Quan, ORourke et al.: "Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase." in: Cell, Vol. 81, Issue 5, pp. 801-9, 1995 (PubMed).

Nicholson, Ali, Thornberry et al.: "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis." in: Nature, Vol. 376, Issue 6535, pp. 37-43, 1995 (PubMed).

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Catalog No. ABIN649434
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