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Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (C-Term) antibody

Details for Product No. ABIN649436
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Antigen
Synonyms xCaspase-6, mch2, caspase-6, zgc:112960, CASP6, casp6, Mch2, mCASP-6, MCH2
Epitope
C-Term
(49), (32), (23), (12), (12), (7), (7), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(196), (92), (77), (13), (6), (2), (1)
Host
Rabbit
(180), (29), (4)
Clonality
Monoclonal
Conjugate
Un-conjugated
(11), (10), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(171), (96), (63), (49), (40), (30), (21), (13), (8), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN649436
450.00 $
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Specificity A synthetic peptide corresponding to residues in C-terminus of human Caspase-6 was used as immunogen. The antibody should recognize both pro-form and p11 subunit.
Alternative Name Caspase-6
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).
Synonyms: CASP6, MCH2, Caspase-6 subunit p11, Apoptotic protease Mch-2
Molecular Weight 34 kDA
Gene ID 839
UniProt P55212
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000 , IHC: = 1:50
Comment

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-6 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Fernandes-Alnemri, Litwack, Alnemri: "Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family." in: Cancer research, Vol. 55, Issue 13, pp. 2737-42, 1995 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Slee, Harte, Kluck et al.: "Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 281-92, 1999 (PubMed).

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