Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (Pro-form) antibody

Antigen

Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)
Synonyms MCH2, Mch2, mCASP-6, MGC93335, xCaspase-6, mch2, caspase-6, CASP6, MGC127633, zgc:112960, casp6
Binding Site
Alternatives

Pro-form
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649437
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name Caspase-6
Gene ID 839
UniProt P55212
Description Other names: CASP6, MCH2, Caspase-6 subunit p11, Apoptotic protease Mch-2
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen. The antibody should recognize the pro-form of Caspase-6.
Molecular Weight 35 kDA
Comments

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: WB: ~~ 1:20000~100000 IP: ~~ 1:50 FC: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Caspase-6 Antibody (Pro) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Takahashi, Alnemri, Lazebnik et al.: "Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8395-400, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6)", type "Antibodies"
Hosts Rabbit (127), Mouse (32)
Reactivities Human (149), Mouse (Murine) (95), Rat (Rattus) (94), Dog (Canine) (22), Cow (Bovine) (5), Rabbit (2), Cat (Feline) (1), Chicken (1), Gerbil (1), Hamster (1), Monkey (1), Pig (Porcine) (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (93), Immunofluorescence (IF) (69), ELISA (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (11), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Flow Cytometry (FACS) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2)
Conjugates Biotin (6), FITC (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes C-Term (46), N-Term (26), pSer257 (22), Internal Region (4), Cleaved-Asp162 (2), active (2), AA 250-264 (1), C-Term,active (1), Center (1), Cleavage Site (1), Middle Region (1), N-Term,AA 16-45 (1), Prodomain (1), Ser257 (1), cleaved (1)