Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6) (pro-form) antibody

Details for Product No. ABIN649437
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Antigen
Synonyms MCH2, Mch2, mCASP-6, xCaspase-6, mch2, caspase-6, zgc:112960, CASP6, casp6
Epitope
pro-form
(41), (27), (22), (10), (7), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(173), (90), (76), (13), (7), (2), (1)
Host
Rabbit
(157), (32), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(8), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(148), (82), (55), (44), (40), (31), (21), (15), (8), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN649437
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Specificity A synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen. The antibody should recognize the pro-form of Caspase-6.
Alternative Name Caspase-6
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).
Synonyms: CASP6, MCH2, Caspase-6 subunit p11, Apoptotic protease Mch-2
Molecular Weight 35 kDA
Gene ID 839
UniProt P55212
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:20000-100000 , IHC: = 1:100-250
Comment

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Caspase-6 Antibody (Pro) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Takahashi, Alnemri, Lazebnik et al.: "Cleavage of lamin A by Mch2 alpha but not CPP32: multiple interleukin 1 beta-converting enzyme-related proteases with distinct substrate recognition properties are active in apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 16, pp. 8395-400, 1996 (PubMed).

Srinivasula, Fernandes-Alnemri, Zangrilli et al.: "The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 43, pp. 27099-106, 1996 (PubMed).

Hosts (157), (32), (2)
Reactivities (173), (90), (76), (13), (7), (2), (1)
Applications (148), (82), (55), (44), (40), (31), (21), (15), (8), (2), (2), (1), (1), (1), (1)
Conjugates (8), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Epitopes (41), (27), (22), (10), (7), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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