|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (Pro-form) antibody
|Synonyms||MCH3, CMH-1, ICE-LAP3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, mch3, cmh-1, ice-lap3, CASP7, LOC100101591|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CASP7, MCH3, Caspase-7 subunit p11, Apoptotic protease Mch-3,CMH-1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7 was used as immunogen. The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.|
|Molecular Weight||34 kDA|
Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000 ICC: ~~ 1:50 IHC: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Caspase-7 Antibody (Pro) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure|
|Restrictions||For Research Use only|
Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).
Lippke, Gu, Sarnecki et al.: "Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 1825-8, 1996 (PubMed).
Duan, Chinnaiyan, Hudson et al.: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).
Chandler, Cohen, MacFarlane: "Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10815-8, 1998 (PubMed).