Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7) (pro-form) antibody

Details for Product No. ABIN649439
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Antigen
Synonyms CASP-7, CMH-1, ICE-LAP3, LICE2, MCH3, Mch3, mCASP-7, AI314680, ICE-IAP3, caspase-7, xCaspase-7, caspase7, cmh-1, ice-lap3, mch3, CASP7
Epitope
pro-form
(19), (16), (9), (8), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(162), (84), (65), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Host
Rabbit
(137), (39), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(150), (72), (50), (49), (20), (20), (15), (8), (7), (4), (3), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN649439
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7 was used as immunogen. The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
Alternative Name Caspase-7
Background Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4).
Synonyms: CASP7, MCH3, Caspase-7 subunit p11, Apoptotic protease Mch-3,CMH-1
Molecular Weight 34 kDA
Gene ID 840
UniProt P55210
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:50
Comment

Background: Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-7 Antibody (Pro) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Fernandes-Alnemri, Takahashi, Armstrong et al.: "Mch3, a novel human apoptotic cysteine protease highly related to CPP32." in: Cancer research, Vol. 55, Issue 24, pp. 6045-52, 1996 (PubMed).

Lippke, Gu, Sarnecki et al.: "Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 1825-8, 1996 (PubMed).

Duan, Chinnaiyan, Hudson et al.: "ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis." in: The Journal of biological chemistry, Vol. 271, Issue 3, pp. 1621-5, 1996 (PubMed).

Chandler, Cohen, MacFarlane: "Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver." in: The Journal of biological chemistry, Vol. 273, Issue 18, pp. 10815-8, 1998 (PubMed).

Hosts (137), (39), (2)
Reactivities (162), (84), (65), (26), (24), (13), (12), (3), (3), (2), (1), (1), (1)
Applications (150), (72), (50), (49), (20), (20), (15), (8), (7), (4), (3), (2), (1), (1)
Conjugates (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (19), (16), (9), (8), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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