Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) antibody

Details for Product No. ABIN649440
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Antigen
Synonyms ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, CASP-8, Mch5, caspase-8, zgc:92075, casp8-A, xCaspase-8, casp8, CASP8
Reactivity
Human, Rat (Rattus)
(202), (70), (57), (28), (13), (12), (12), (9), (4), (2), (1), (1)
Host
Rabbit
(174), (54), (6), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(201), (84), (50), (48), (31), (30), (15), (15), (11), (10), (4), (3), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN649440
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18 was used as immunogen. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
Alternative Name Caspase-8
Background Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
Synonyms: CASP8, MCH5, Caspase-8 subunit p10, Apoptotic cysteine protease,Apoptotic protease Mch-5,CAP4
Molecular Weight 55 kDA
Gene ID 841
UniProt Q14790
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500
Comment

Background: Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-8 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Boldin, Goncharov, Goltsev et al.: "Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death." in: Cell, Vol. 85, Issue 6, pp. 803-15, 1996 (PubMed).

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997 (PubMed).

Hosts (174), (54), (6), (1)
Reactivities (202), (70), (57), (28), (13), (12), (12), (9), (4), (2), (1), (1)
Applications (201), (84), (50), (48), (31), (30), (15), (15), (11), (10), (4), (3), (2), (2), (2), (2), (1), (1)
Conjugates (9), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (29), (12), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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