Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) antibody

Antigen: Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8)

Synonyms: CAP4, MACH, MCH5, FLICE, ALPS2B, Casp-8, FLJ17672, MGC78473, Mch5, Caspase-8, MGC92075, caspase-8, zgc:92075, casp8-A, xCaspase-8, CASP8, casp8, DKFZp469A1428

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649440

Additional Information

Alternative name: Caspase-8

Gene ID: 841

UniProt: Q14790

Description: Other names: CASP8, MCH5, Caspase-8 subunit p10, Apoptotic cysteine protease,Apoptotic protease Mch-5,CAP4

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18 was used as immunogen. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.

Molecular Weight: 55 kDA

Comments:

Background: Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:40 FC: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Caspase-8 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Boldin, Goncharov, Goltsev et al.: "Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death." in: Cell, Vol. 85, Issue 6, pp. 803-15, 1996 (PubMed).

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997 (PubMed).