Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (N-Term) antibody

Details for Product No. ABIN649441
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Antigen
Synonyms casp8-A, xCaspase-8, casp8, CASP8, CASP-8, FLICE, MACH, Mch5, ALPS2B, CAP4, Casp-8, MCH5, caspase-8, zgc:92075
Epitope
N-Term
(30), (24), (10), (7), (7), (6), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(239), (80), (60), (25), (13), (12), (12), (9), (5), (2), (1), (1)
Host
Rabbit
(206), (56), (7), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(237), (99), (53), (52), (36), (26), (16), (16), (14), (10), (9), (6), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN649441
450.00 $
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Specificity A synthetic peptide corresponding to N-terminal residues of human Caspase-8 was used as immunogen. The antibody should recognize all splice isoforms of Caspase-8.
Alternative Name Caspase-8
Background Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.
Synonyms: CASP8, MCH5, Caspase-8 subunit p10, Apoptotic cysteine protease,Apoptotic protease Mch-5,CAP4
Molecular Weight 55 kDA
Gene ID 841
UniProt Q14790
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Application Notes IHC: = 1:100, WB: = 1:500-1000
Comment

Background: Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Caspase-8 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Boldin, Goncharov, Goltsev et al.: "Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death." in: Cell, Vol. 85, Issue 6, pp. 803-15, 1996 (PubMed).

Vincenz, Dixit: "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6578-83, 1997 (PubMed).

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