CD90 (THY1) antibody

Antigen: CD90 (THY1)

Synonyms: CD90, FLJ33325

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649513

Additional Information

Alternative name: CD90

Gene ID: 6657

UniProt: P04216

Description: Other names: THY1, Thy-1 membrane glycoprotein, Thy-1 antigen,CDw90

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the N-terminal of human CD90 was used as an immunogen.

Molecular Weight: 25-35 kDA

Comments:

Background: CD90 (Cluster of Differentiation 90, Thy-1) is a heavily N-glycosylated GPI linked cell surface protein member of the immunoglobulin (Ig) superfamily. As a smallest member of family, CD90 may play a role in cell-to-cell and cell-to-ligand interactions during synaptogensis and other neuron related activities (e.g. neurite outgrowth, nerve regeneration) (1). In developing nervous tissue, CD90 is involved in axon growth regulation by halting axon growth in mature axon via its interaction with intergin-beta-3 on mature astrocytes. Additionally, CD90 has been linked to apoptosis, adhesion, migration, cancer (including cancer stem cells), and fibrosis (2). As a major marker for hematopoietic stem cells (HSCs), CD90 mediates differentiation of HSCs. In stem cancer cell research, CD90 positive cells in the hepatocellular carcinoma (HCC) have been shown to display tumorigenic capacity (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: CD90 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Rege, Hagood: "Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 8, pp. 1045-54, 2006 (PubMed).

"The universal protein resource (UniProt)." in: Nucleic acids research, Vol. 36, Issue Database issue, pp. D190-5, 2008 (PubMed).

Yang, Ho, Ng et al.: "Significance of CD90+ cancer stem cells in human liver cancer." in: Cancer cell, Vol. 13, Issue 2, pp. 153-66, 2008 (PubMed).